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低温胁迫下苦瓜苗期生理变化与耐冷性
评价的关系分析
作者: 陈小凤1 2 黄如葵1 2 * 黄玉辉1 2 冯诚诚1 2 黄熊娟1 梁家作1 刘杏连1 
单位: (1 广西农业科学院 蔬菜研究所 南宁 530007 2 广西作物遗传改良生物技术重点开放实验室 南宁 530007) 
关键词: 苦瓜 低温胁迫 生理指标 耐冷性评价 
分类号:S642.5
出版年,卷(期):页码:2017,48(7):1237-1241
摘要:

【目的】分析低温胁迫下苦瓜苗期生理变化与耐冷性评价的关系,筛选适宜苦瓜苗期耐冷性评价的取材时期,为提高苦瓜耐冷性评价的准确性和可靠性提供参考依据。【方法】对已知耐冷性存在差异的苦瓜材料MC108、MC45和MC6幼苗进行10 ℃低温胁迫,分别测定其叶片在胁迫0、2、4、6、8和10 d时的生理指标,绘图显示生理指标的变化情况,结合隶属函数值对各胁迫时期苦瓜耐冷性的评价结果,筛选适宜苦瓜苗期耐冷性评价的取材时期。【结果】耐冷性存在差异的苦瓜材料对冷害胁迫反应机制不同,与低温胁迫0 d(对照)相比,低温胁迫第10 d时冷敏材料MC6的可溶性蛋白含量降幅较大,脯氨酸(Pro)含量增加量更多;与MC45和MC6相比,低温胁迫0、2、4、6、8和10 d时耐冷材料MC108的超氧化物歧化酶(SOD)和过氧化物酶(POD)活性变幅相对较小。除丙二醛(MDA)含量峰值出现的时间不同外,MC108、MC45和MC6的Pro、可溶性蛋白含量及SOD、POD活性降低的共同时间点均为低温胁迫处理第10 d。结合隶属函数值对苦瓜耐冷性的评价结果判断,低温胁迫处理第10 d的叶片生理指标适宜作为其苗期耐冷性评价指标。【结论】苦瓜材料幼苗进行10 ℃低温胁迫处理第10 d是进行苗期耐冷性评价的适宜取材时期。

【Objective】Relation between physiological change and chilling tolerance of bitter gourd at seedling stage under cold temperature was analyzed, the suitable sampling time for chilling tolerance evaluation was selected in order to providing references for the accuracy and reliability of evaluating chilling tolerance. 【Method】Bitter gourd materials MC108, MC45 and MC6 seedlings, whose chilling tolerances were various, were stressed at 10 ℃. The physiological indexes were measured on leaves at 0,2,4,6,8 and 10 d under low temperature stress. The changes of physiological indexes of materials were drew. Combining the chilling tolerance evaluation results of subordinate function values at different stress times, the suitable sampling time for bitter gourd chilling tolerance evaluation at seedling stage was selected. 【Result】The response mechanisms varied in bitter gourd with different chilling tolerances under low temperature stress. Compared with 0 d under low temperature stress(control), soluble protein content decrease of cold sensitive material MC6 at 10 d of stress was larger, proline(Pro) content increase of MC6 was larger. Compared with MC45 and MC6, the change range of superoxide dismutase(SOD) and peroxidase(POD) activities in cold tolerant material MC108 at 2,4,6,8 and 10 d under chilling stress was smaller. Apart from malondialdehyde(MDA) content peak, the times of Pro content, soluble protein content, SOD activity and POD activity decrease of MC108, MC45 and MC6 were day 10 under low temperature stress. Combining the chilling tolerance evaluation results of subordinative function values, leaf physiological indexes on day 10 under low temperature stress was the suitable for chilling tolerance evaluation at seedling stage. 【Conclusion】Bitter gourd seedlings under 10 ℃ low temperature stress for 10 d is the suitable sampling time for chilling tolerance evaluation.

基金项目:
广西科学研究与技术开发计划项目(桂科合14125008-2-19) ;广西农业科学院科技发展基金项目(桂农科2017JM44);广西农业科学院基本科研业务专项项目(桂农科2014YQ17);现代农业产业技术体系建设专项项目(CARS-25)
作者简介:
*为通讯作者,黄如葵(1969-),研究员,主要从事蔬菜遗传育种及栽培研究工作,E-mail:rkhuang@gxaas.net。陈小凤(1975-),副研究员,主要从事蔬菜遗传育种及栽培研究工作,E-mail:cxf@gxaas.net
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