首页 学报新闻学报概况编委会专家库学术影响投稿指南审核流程标准规范下载专区投诉邮箱联系我们EngLish
 位置:首页 >> 正文
PAHs胁迫对菜心品质及其解毒系统的影响
作者: 龙明华1 巫桂芬1 梁勇生1 2 申海燕1  彪1  璇1 李朋欣1 张慧娜1 3 
单位: (1 广西大学 南宁 530004 2 南宁市蔬菜研究所 南宁 530000 3 广西农业职业技术学院 南宁 530007) 
关键词: 菜心 PAHs 品质 酶活 解毒系统 
分类号:S634.5
出版年,卷(期):页码:2017,48(6):1036-1041
摘要:

【目的】探讨多环芳烃(PAHs)胁迫对菜心品质及其解毒系统的影响,了解PAHs对菜心的胁迫机理,为保障蔬菜质量安全提供参考依据。【方法】利用16种PAHs胁迫菜心,检测各处理菜心的单株鲜重、叶绿素、可溶性糖、可溶性蛋白、维生素C(Vc)和粗纤维含量及超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)、过氧化物酶(POD)和多酚氧化酶(PPO)活性的变化情况,分析各品质指标和相关酶活性与PAHs的关系。【结果】900.0 μg/L PAHs胁迫使菜心单株鲜重降低12.65%;300.0~900.0 μg/L PAHs胁迫使菜心Vc和叶绿素含量降低,粗纤维含量增加,导致色泽改变、口感变差,但可增加菜心的可溶性蛋白含量,启动菜心机体免疫系统;低浓度(300.0 μg/L)PAHs可致菜心的可溶性糖含量降低,口感变差。PAHs胁迫使菜心相关酶活性发生上调或下调作用,进而解除PAHs胁迫,其中,SOD活性降低;CAT活性呈先降低后升高变化趋势,但升幅或降幅小于对照(CK);POD活性随着胁迫时间延长而降低,表明CAT与POD在功能上共同承担了部分解毒功能;600.0 μg/L PAHs可促进APX活性增强;PPO活性呈先升后降变化趋势,有利于机体实现自我修复过程。【结论】在PAHs胁迫下,菜心品质变差;低浓度(300.0 μg/L)PAHs胁迫可提高其CAT和POD活性,中浓度(600.0 μg/L)PAHs胁迫有利于提高SOD活性,以降低蔬菜体内PAHs积累。

【Objective】Effects of polycyclic aromatic hydrocarbons(PAHs) stress on Brassica parachinensis Bailey quality and its detoxification system were discussed to study the stress mechanism of PAHs on B. parachinensis Bailey and provide reference basis to ensure B. parachinensis Bailey quality.【Method】Using 16 kinds of PAHs to stress B. parachinensis Bailey, variations of single plant fresh weight, chlorophyll, soluble sugar, soluble protein, vitamin C(Vc), crude fiber and superoxide dismutase(SOD), catalase(CAT), ascorbate peroxidase(APX), peroxidase(POD) and polyphenol oxidase(PPO) activities were tested. After that, the quality indicators and related enzymes activities and their relationships with PAHs were analyzed. 【Result】900.0 μg/L PAHs stress decreased single plant fresh weight by 12.65%. 300.0-900.0 μg/L PAHs lowered Vc and chlorophyll contents, but increased  crude fiber content in B. parachinensis Bailey. In this way, PAHs changed the color and made the taste worse, but it could increase the soluble protein content and start the immune system of B. parachinensis Bailey. Low concentration(300.0 μg/L) of PAHs could cause vegetable soluble sugar content lower and the taste worse. PAHs stress made the related enzymes activities up- or down-regulated to remove PAHs stress. SOD activity decreased;CAT activity went through a down-up variation, but the variation range was smaller than that of control(CK); POD activity decreased as stress time extended, which indicated that CAT and POD shared partial detoxification function. 600.0 μg/L PAHs could promote APX activity; PPO activity increased first and then declined, which was be-
neficial for self-healing process. 【Conclusion】Under the stress of PAHs, the quality of B. parachinensis Bailey is worse. Low concentration(300.0 μg/L) of PAHs stress improves CAT and POD activities. Medium concentration(600.0 μg/L)of PAHs stress is beneficial for improving SOD activity to lower PAHs accumulation in B. parachinensis Bailey.

基金项目:
国家自然科学基金项目(31360479);广西自然科学基金项目(2014GXNSFAA118100);国家现代农业产业技术体系广西大宗蔬菜创新团队建设项目(nycytxgxcxtd-03-10-1)
作者简介:
龙明华(1961-),教授,主要从事蔬菜育种及农产品质量安全研究工作,E-mail:longmhua@163.com
参考文献:
服务与反馈:
文章下载】【加入收藏
总浏览数:10255320 今日总人数: 4535
主办:广西农业科学院    地址:广西南宁市大学东路174号   邮政编码:530007  
电话:(+86)-771-3243905; (+86)-771-3244920   电子邮箱:gxny@163.net  
Copyright © 2011 Nfnyxb.com, All Rights Reserved  版权所有©2011《南方农业学报》编辑部