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玉米WRKY基因功能分析
作者: 决登伟1 2 桑雪莲1 2  波1 2 刘丽琴1 2 王一承1 2 石胜友1 2 * 
单位: (1 中国热带农业科学院 南亚热带作物研究所 广东 湛江 524091 2 农业部热带果树生物学重点实验室 广东 湛江 524091) 
关键词: 玉米 WRKY转录因子 功能分析 非生物胁迫 
分类号:S512
出版年,卷(期):页码:2017,48(6):945-951
摘要:

【目的】分析WRKY基因在玉米生长发育及逆境胁迫下的功能,为阐明WRKY家族基因在玉米生长和逆境响应机制中的功能和作用打下基础。【方法】利用生物信息学技术从玉米基因组中得到3个进化关系较近的WRKY基因,应用在线预测软件对3个基因的功能和结构进行分析,并运用荧光定量RT-PCR(qRT-PCR)分析3个基因在玉米不同组织及在高盐、低温和干旱胁迫下的表达模式。【结果】从玉米基因组中得到ZmWRKY22-like、ZmWRKY55-like和ZmWRKY74-like 3个玉米WRKY转录因子家族基因,预测表明3个蛋白均定位于细胞核。荧光定量PCR分析结果表明,3个基因在玉米的不同器官中均有表达,但具有组织表达特异性。高盐处理24 h,ZmWRKY55-like基因的表达量上升为对照的4.5倍;低温处理24 h,ZmWRKY74-like基因的表达量上升为对照的2.1倍。【结论】ZmWRKY22-like、ZmWRKY55-like和ZmWRKY74-like基因可能在玉米果实发育过程中起到一定作用,其中ZmWRKY55-like和ZmWRKY74-like基因可能分别参与植物对盐和低温胁迫的响应。

【Objective】The function of maize WRKY gene in growth and stress was studied, in order to lay a foundation for researching the function of WRKY family genes in the growth and response mechanisms of stress in maize. 【Method】 Three WRKY genes which showed a close evolutionships in maize genome were found by using bioinformatics technology. Meanwhile, the structure and function of those ZmWRKY genes were analyzed. In addition, the expression levels of ZmWRKY genes in different maize tissues and under high salt, low temperature and drought stress were analyzed by fluore-
scence quantitative RT-PCR(qRT-PCR). 【Result】Three WRKY transcription factor genes(ZmWRKY22-like,ZmWRKY55-
like and ZmWRKY74-like) in maize were identified.  It was predicted that these three proteins were all localized in nucleus. Fluorescence quantitative PCR assay showed that ZmWRKY22-like,ZmWRKY55-like and ZmWRKY74-like expressed in all tested maize tissues and had  differential expressions. The expression of ZmWRKY55-like was up-regulated about 4.5 times under high salinity treatment for 24 h and that of ZmWRKY74-like was up-regulated about 2.1 times under low temperature stress. 【Conclusion】ZmWRKY22-like, ZmWRKY55-like and ZmWRKY74-like genes might play a role in maize fruit growth. ZmWRKY55-like and ZmWRKY74-like may be involved into plant response to salt and low temperature stress respectively.

基金项目:
国家自然科学基金项目(31572087);海南省自然科学基金项目(20163111)
作者简介:
*为通讯作者,石胜友(1970-),博士,研究员,主要从事植物基因工程研究工作,E-mail: ssy7299@163.com。决登伟(1986-),博士,主要从事植物基因工程研究工作,E-mail: juedengwei@126.com
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