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六堡茶中金花菌的分离与鉴定
作者: 欧惠算1 邓旭铭1 张灵枝1 * 张均伟2 马士成3 4 邱瑞瑾3 4 
单位: (1 华南农业大学 园艺学院 广州 510642 2 梧州中茶茶业有限公司 广西 梧州 543001 3 梧州市农业科学研究所  
关键词: 六堡茶 金花菌 阿姆斯特丹散囊菌 分离 鉴定 
分类号:S571.1
出版年,卷(期):页码:2017,48(4):658-662
摘要:

【目的】研究鉴定六堡茶中的金花菌,为推动六堡茶产业发展提供一定的理论基础。【方法】采用平板涂布法及三点接种法从梧州中茶茶厂2015年11月生产的六堡茶中分离纯化金花菌,以传统的形态学观察法观察菌落特征,利用光学显微镜观察菌丝、分生孢子等结构特征,再结合分子生物学方法对分离获得的菌株进行DNA序列分析,并通过ITS序列进行同源性搜索比对和系统发育进化树分析。【结果】分离获得一株金花菌,命名为LB-1。LB-1菌株的形态特征与散囊菌属很相似,主要表现为:菌落呈金黄色、有同心环、有放射性脊等,其ITS序列与阿姆斯特丹散囊菌(Eurotium amstelodami)的同源性达99%,进一步确定LB-1菌株为阿姆斯特丹散囊菌。【结论】从六堡茶中分离获得的金花菌LB-1为散囊菌属中的阿姆斯特丹散囊菌。在六堡茶加工中,可为LB-1菌提供适宜的环境,促进其大量繁殖,以增强六堡茶的保健功效。

【Objective】Jinhua fungus in Liupao tea was identified in order to provide theoretical basis for Liupao tea industry development. 【Method】Spread-plate method and three-point inoculation method were adopted to isolate and purify Jinhua fungus from Liupao tea produced by China Tea(Wuzhou) Factory in November, 2015. The colony characteristics were observed by traditional morphology observation method, and the structures like hypha and conidium were observed via optical microscope. Then DNA sequence analysis for strain isolated was conducted combining molecular biology method, and  homology searching and comparison via ITS sequence and phylogenetic tree analysis were carried out. 【Result】One strain of Jinhua fungus was isolated and named LB-1. The morphological characteristics of LB-1were similar to those of Eurotium: golden colony, concentric rings, radioactive ridge, etc. The homology of its ITS sequence to Eurotium amstelodamiwas 99%. Therefore, LB-1 was identified as E. amstelodami. 【Conclusion】Jinhua fungus LB-1 is isolated from Liupao tea, and identified as E. amstelodami in Eurotium. During processing of Liupao tea, the producers can create suitable environment for LB-1 and promote the mass reproduction of LB-1, so as to enhance the health care effects of Liupao tea.

基金项目:
广东省自然科学基金项目(5300-E12237);梧州市科学研究与技术开发计划项目(201501024)
作者简介:
*为通讯作者,张灵枝(1972-),博士,副教授,主要从事茶叶深加工与综合利用研究工作,E-mail:lingzhi@scau.edu.cn。欧惠算(1991-),研究方向为茶叶生物化学与深加工综合利用,E-mail:781088344@qq.com
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