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利用SSR分子标记技术鉴定和分析
茄子杂种F1的纯度
作者: 王艳娜1 2 3 王益奎2 3 4 李文嘉1 2 * 蒋雅琴2 吴永官2  炎2 康德贤2 
单位: (1 广西大学 农学院 南宁 530005 2 广西农业科学院 蔬菜研究所 南宁 530007 3 广西作物遗传改良生物技术重点开放实验室 南宁 530007 4 华南农业大学 园艺学院 广州 510642) 
关键词: 茄子 SSR分子标记技术 杂种纯度 鉴定 亲缘关系分析 
分类号:S641.1
出版年,卷(期):页码:2015,46(9):1551-1556
摘要:

【目的】分析、鉴定茄子杂种F1纯度,为提高茄子杂交育种效率提供参考依据。【方法】在育苗期,采用SSR分子标记技术对栽培茄×栽培茄正、反杂交种和野生茄×栽培茄杂交种进行纯度鉴定及亲缘关系分析。【结果】从124对SSR引物中筛选出15对在亲本间存在差异、扩增稳定、条带清晰的引物;从210株杂种F1单株中共鉴定出208株真杂种,杂种纯度为99.0%,鉴定结果与田间性状调查结果一致;栽培茄×栽培茄正、反交组合的F1与亲本的亲缘关系均较近,遗传关系差别微小;野生茄×栽培茄组合的F1与父本的亲缘关系均较远,且表现出偏母本遗传。【结论】SSR分子标记技术能快速、准确鉴定茄子杂交后代的纯度,可作为分子标记辅助育种手段用于指导选择符合茄子育种目标的优良杂交品种选育。

【Objective】The F1 hybrid purity of eggplant was identified and analyzed,in order to provide reference for improving cross breeding efficiency of eggplant. 【Method】The SSR molecular marker technology was used to identify hybrid purity and analyse genetic relationship for F1 hybrids of Solanum melongena×Solanum melongena reciprocal-crossing combination and S. integrifolium×S. melongena combination. 【Result】The results showed that 15 pairs of SSR primers were screened out from 124 pairs of SSR primers, which could stably amplify clear differential bands between parents. As to 210 plants of F1 hybrids, 208 plants were identified as true hybrid, with hybrid rate of 99.0%, and this result was consistent with investigation result of characters in the field. In addition, the F1 hybrids of S. melongena×S. melongena reciprocal-crossing combination had closer genetic relationship with parents with subtle difference. However, the F1 hybrids of S. integrifolium×S. melongena combination had rather distant genetic relationship with male parent, which preferred to maternal inheritance. 【Conclusion】The SSR molecular marker technology is rapid and accurate, which can be used to identify eggplant hybrid. Furthermore, the SSR molecular marker can be used as an auxiliary means to breed improved hybrid varieties.

基金项目:
上海市设施园艺技术重点实验室开放基金项目(SS2013KF02)
作者简介:
*为通讯作者,李文嘉(1962-),主要从事茄子与番茄育种研究工作,E-mail:25672005@qq.com。王艳娜(1988-),研究方向为分子遗传育种,E-mail:916468005@qq.com
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